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Chemical Sciences
Introduction
Objective
List of experiments
Target Audience
Course Alignment
Feedback
Introduction
Objective
List of experiments
Target Audience
Course Alignment
Feedback
Circular Dichronism Spectroscopy
To determine the specific rotation of a sugar using a polarimeter
To understand the effect of chiral substances on plane polarized light as a function of wavelength
To study the Optical Rotatory Dispersion (ORD) of some chiral substances
To study the Circular Dichroism of some substances with chiral chromophores
To Study the operational details of a CD spectrometer
To Study the effect of Secondary Structure elements on CD spectrum of proteins
To deconvolute the CD spectrum of a given protein solution and to classify it in terms of its secondary structure elements
To study the thermal stability of proteins
To study the effect of chemical denaturants on protein stability
To study the comparison between protein unfolding and protein aggregation in the presence of chemical denaturing agents
